Publications [#234178] of Eric J. Toone

Journal Articles

1. Wilfong, EM; Locklear, U; Toone, EJ, A single step purification for autolytic zinc proteinases., Bioorganic & medicinal chemistry letters, vol. 20 no. 1 (January, 2010), pp. 280-282 [19942433], [doi]
   (last updated on 2026/01/24)

   Abstract:
   We describe a novel single-step method for the purification of stromelysin-1 catalytic domain (SCD) via immobilized metal affinity chromatography under denaturing conditions that inhibit proteolytic activity followed by on-column refolding and spontaneous autolysis of the fusion peptide to yield pure, active stromelysin-1 catalytic domain. The methodology provides a general approach for the rapid purification of large quantities of zinc proteinases.