Publications [#112797] of G Vann V. Bennett

Papers Published

1. LH Davis, V Bennett, Identification of two regions of beta G spectrin that bind to distinct sites in brain membranes., The Journal of biological chemistry, UNITED STATES, vol. 269 no. 6 (February, 1994), pp. 4409-16, ISSN 0021-9258
   (last updated on 2003/02/18)

   Abstract:
   This study analyzed the complex interactions of intact spectrin with bovine brain membranes by evaluating membrane associations of defined regions of beta G spectrin, the subunit responsible for high affinity membrane binding. Two regions of beta G spectrin were expressed in bacteria and demonstrated to contain fully functional binding site(s) for a subset of spectrin-binding sites in brain membranes depleted of peripheral proteins. One region, located near the NH2 terminus, was comprised of 106-residue repeats and required repeats 2-7 for full activity. The other binding domain was located at the COOH terminus, which is the most variable between beta G and beta R spectrins, is distinct from the 106-residue repeats, and contains a pleckstrin homology domain. NH2-terminal beta spectrin polypeptides interacted with a membrane site(s) that recognized both brain and erythrocyte isoforms of spectrin, was inhibited by calcium/calmodulin, and was not blocked by the COOH-terminal polypeptide. The COOH-terminal region associated with a membrane site(s) that was specific for brain spectrin, was not inhibited by calcium/calmodulin, and was not blocked by the NH2-terminal polypeptide. These observations demonstrate membrane association of spectrin with at least two independent sites, which differ with regard to regulation by calcium/calmodulin and in selectivity for spectrin isoforms.

   Keywords:
   Animals • Ankyrins • Binding Sites • Binding, Competitive • Brain • Cattle • Cell Membrane • Peptide Fragments • Recombinant Proteins • Repetitive Sequences, Nucleic Acid • Spectrin • Structure-Activity Relationship • chemistry • metabolism • metabolism*