Publications [#112814] of G Vann V. Bennett

Papers Published

1. E Kordeli, S Lambert, V Bennett, AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier., The Journal of biological chemistry, vol. 270 no. 5 (February, 1995), pp. 2352-9, ISSN 0021-9258
   (last updated on 2013/05/16)

   Abstract:
   We have characterized a new ankyrin gene, expressed in brain and other tissues, that is subject to extensive tissue-specific alternative mRNA processing. The full-length polypeptide has a molecular mass of 480 kDa and includes a predicted globular head domain, with membrane- and spectrin-binding activities, as well as an extended "tail" domain. We term this gene ankyrinG based on its giant size and general expression. Two brain-specific isoforms of 480 kDa and 270 kDa were identified that contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following the globular head domain. Antibodies against the serine-rich domain and spectrin-binding domain revealed labeling of nodes of Ranvier and axonal initial segments. Ankyrin-binding proteins also known to be localized in these specialized membrane domains include the voltage-dependent sodium channel, the sodium/potassium ATPase, sodium/calcium exchanger, and members of the neurofascin/L1 family of cell adhesion molecules. The neural-specific ankyrinG polypeptides are candidates to participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments.

   Keywords:
   Alternative Splicing • Amino Acid Sequence • Animals • Ankyrins • Axons • Base Sequence • Cerebral Cortex • Fluorescent Antibody Technique • Gene Expression • Genes • Humans • Molecular Sequence Data • Nerve Tissue Proteins • RNA, Messenger • Ranvier's Nodes • Rats • Sequence Alignment • Sequence Homology, Amino Acid • Tissue Distribution • chemistry • chemistry* • genetics • immunology • metabolism* • ultrastructure