Publications [#112903] of G Vann V. Bennett

Papers Published

1. SM Jenkins, V Bennett, Developing nodes of Ranvier are defined by ankyrin-G clustering and are independent of paranodal axoglial adhesion., Proceedings of the National Academy of Sciences of the United States of America, vol. 99 no. 4 (February, 2002), pp. 2303-8, ISSN 0027-8424 [doi]
   (last updated on 2013/05/16)

   Abstract:
   Nodes of Ranvier are excitable regions of axonal membranes highly enriched in voltage-gated sodium channels that propagate action potentials. The mechanism of protein clustering at nodes has been a source of controversy. In this study, developmental analysis of nodes of Ranvier in optic nerve axons reveals that early node intermediates are defined by ankyrin-G. Other node components, including beta IV spectrin, voltage-gated sodium channels, and the L1 cell adhesion molecule neurofascin, are subsequently recruited to sites of ankyrin-G clustering. The role of intact paranodes in protein clustering was examined in the dysmyelinating mouse mutant jimpy. Jimpy mice do not have intact paranodal axoglial contacts, which is indicated by a complete lack of neurexin/contactin-associated protein/paranodin clustering in paranodes. In the absence of intact paranodes, ankyrin-G was still able to cluster, although fewer ankyrin clusters were seen in jimpy optic nerves than in wild-type optic nerves. Recruitment of Na(v)1.2, Na(v)1.6, beta IV spectrin, and neurofascin to sites of ankyrin-G clustering is unimpaired in jimpy mice, indicating that node formation occurs independent of intact paranodal axoglial contacts.

   Keywords:
   Amino Acid Sequence • Animals • Ankyrins • Axons • Cell Adhesion • Cell Adhesion Molecules • Immunohistochemistry • Mice • Mice, Jimpy • Microscopy, Fluorescence • Myelin Sheath • Nerve Growth Factors • Neuroglia • Optic Nerve • Peptides • Ranvier's Nodes • Rats • Sodium Channels • biosynthesis • biosynthesis* • chemistry • chemistry* • metabolism • metabolism*