Publications [#112946] of G Vann V. Bennett

Papers Published

1. M Tsirigotis, N Nicholson, M Taranissi, V Bennett, M Pelekanos, I Craft, Late intracytoplasmic sperm injection in unexpected failed fertilization in vitro: diagnostic or therapeutic?, Fertility and sterility, UNITED STATES, vol. 63 no. 4 (April, 1995), pp. 816-9, ISSN 0015-0282
   (last updated on 2003/02/18)

   Abstract:
   OBJECTIVE: To evaluate fertilization potential of 24-hour-old unfertilized oocytes using intracytoplasmic sperm injection and the pregnancy potential of resultant embryos. DESIGN: Prospective observational study. SETTING: Private infertility clinic, London, United Kingdom. PATIENTS: Fifteen patients with a history of infertility who underwent treatment with IVF and showed failure of fertilization on the day after oocyte retrieval. INTERVENTION: Assisted fertilization with intracytoplasmic sperm injection was carried out at 24 hours after oocyte retrieval. RESULTS: A total of 121 metaphase II oocytes were subjected to intracytoplasmic sperm injection. Of these, 9 were damaged (7%), 2 were polyploidic (2%), and 58 showed normal fertilization (48%). Of the latter, 47 cleaved normally (81%). Forty embryos were transferred and three were cryopreserved. One patient conceived (7%) but in this case only one of three embryos transferred was from intracytoplasmic sperm injection. CONCLUSION: Late (24 hours) intracytoplasmic sperm injection can give good fertilization and cleavage rates but the potential of the generated embryos to achieve pregnancy seems to be low.

   Keywords:
   Adult • Cleavage Stage, Ovum • Cytoplasm • Embryo Transfer • Female • Fertilization in Vitro • Humans • Male • Microinjections • Pregnancy • Prospective Studies • Spermatozoa* • Time Factors • Treatment Failure • methods*