Publications [#98385] of John H Sampson

Papers Published

1. A Lal, CA Glazer, HM Martinson, HS Friedman, GE Archer, JH Sampson, GJ Riggins, Mutant epidermal growth factor receptor up-regulates molecular effectors of tumor invasion., Cancer research, vol. 62 no. 12 (June, 2002), pp. 3335-9, ISSN 0008-5472
   (last updated on 2013/05/13)

   Abstract:
   The gene most commonly altered in human glioblastomas is the epidermalgrowth factor receptor (EGFR). We profiled transcripts induced by mutantEGFR to better understand its role in tumor progression. The pattern found suggested enhanced tumor invasion. The highly induced genes included extracellular matrix components, metalloproteases, and a serine protease. We confirmed that mutant EGFR did make glioblastoma cells both more motile and invasive using in vitro assays. Furthermore, inhibitors of EGFR (OSI-774 and Tyrphostin AG1478) selectively down-regulated these molecular effectors in glioblastoma cells, eliminating enhanced invasion.

   Keywords:
   Animals • Enzyme Inhibitors • Extracellular Matrix Proteins • Gene Expression Profiling • Gene Expression Regulation, Neoplastic • Glioblastoma • Humans • Metalloendopeptidases • Mice • Mice, Nude • Neoplasm Invasiveness • Quinazolines • Receptor, Epidermal Growth Factor • Reverse Transcriptase Polymerase Chain Reaction • Serine Endopeptidases • Transfection • Tumor Cells, Cultured • Tyrphostins • Up-Regulation • antagonists & inhibitors • biosynthesis • drug effects • genetics • genetics* • metabolism • pathology • pharmacology • physiology*