Publications [#98423] of John H Sampson

Papers Published

1. DM Ashley, JH Sampson, GE Archer, SK Batra, DD Bigner, LP Hale, A genetically modified allogeneic cellular vaccine generates MHC class I-restricted cytotoxic responses against tumor-associated antigens and protects against CNS tumors in vivo., Journal of neuroimmunology, NETHERLANDS, vol. 78 no. 1-2 (September, 1997), pp. 34-46, ISSN 0165-5728
   (last updated on 2006/06/06)

   Abstract:
   An active immunotherapeutic strategy using transfected allogeneic cells for targeting the mutant epidermal growth factor receptor (EGFRvIII) on intracranial tumors was examined. Immunization with allogeneic 300.19/EGFRvIII cells induced CD8+ cytotoxic T-lymphocytes against EGFRvIII bearing syngeneic B16-F10 melanoma or 560 astrocytoma cells (H-2b), but not against allogeneic NR6 cells (H-2q) also bearing EGFRvIII significant NK cell activity was also noted in vitro. Vaccination protected against intracranial challenge with EGFRvIII-positive tumor, with 50% long term survival. In vivo depletions of effector cell subsets demonstrated the requirements for both CD8+ and CD4+ T-cells but not NK cells in producing this protective effect. These data demonstrate the generation of significant, antigen-specific and MHC class I-restricted cytotoxic immune responses which are effective against tumors present in the CNS.

   Keywords:
   Animals • Antigens, Neoplasm • Cancer Vaccines • Central Nervous System Neoplasms • Cytotoxicity, Immunologic* • Female • Genetic Techniques • Histocompatibility Antigens Class I • Immunization • Mice • Mutation • Neoplasm Transplantation • Receptor, Epidermal Growth Factor • Survival Analysis • Transfection • Tumor Cells, Cultured • genetics • immunology • immunology* • metabolism • prevention & control*