Publications [#64514] of Bruce M. Klitzman

Papers Published

1. Schoenfisch, M.H. and Nablo, B.J. and Prichard, H.L. and Butler, R.D. and Klitzman, B., Inhibition of implant-associated infections via nitric oxide release, Biomaterials (UK), vol. 26 no. 34 (2005), pp. 6984 - 90 [017]
   (last updated on 2007/04/15)

   Abstract:
   The in vivo antibacterial activity of nitric oxide (NO)-releasing xerogel coatings was evaluated against an aggressive subcutaneous Staphylococcus aureus infection in a rat model. The NO-releasing implants were created by coating a medical-grade silicone elastomer with a sol-gel-derived (xerogel) film capable of storing NO. Four of the bare or xerogel-coated silicone materials were subcutaneously implanted into male rats. Ten rats were administered 10μl of a 10⁸cfuml^-1S. aureus colony directly into the subcutaneous pocket with the implant prior to wound closure. Infection was quantitatively and qualitatively evaluated after 8d of implantation with microbiological and histological methods, respectively. A 82% reduction in the number of infected implants was achieved with the NO-releasing coating. Histology revealed that the capsule formation around infected bare silicone rubber controls was immunoactive and that a biofilm may have formed. Capsule formation in response to NO-releasing implants had greater vascularity in comparison with uninoculated or untreated controls. These results suggest that NO-releasing coatings may dramatically reduce the incidence of biomaterial-associated infection. [All rights reserved Elsevier]

   Keywords:
   aerogels;biological tissues;coatings;elastomers;microorganisms;nitrogen compounds;prosthetics;silicone rubber;sol-gel processing;