Publications [#232622] of Michael C. Fitzgerald

Journal Articles

1. Yang, X; Wang, M; Fitzgerald, MC, Analysis of protein folding and function using backbone modified proteins., Bioorganic chemistry, vol. 32 no. 5 (October, 2004), pp. 438-449, ISSN 0045-2068 [15381405], [doi]
   (last updated on 2026/01/15)

   Abstract:
   With the recent development of chemical and biological methods to introduce backbone modifications into the polypeptide chains of proteins, there have been a growing number of site-directed mutagenesis experiments focused on understanding the role of the polypeptide backbone in protein folding and function. The substitution of a main chain amide bond with an ester bond is now a popular mutation to investigate the role of the polypeptide backbone in ligand, binding, enzyme catalysis, and protein folding. Here we review the results of studies on some 25 ester-bond containing analogues from nine different protein systems. The structural, thermodynamic, and functional consequences of introducing backbone amide- to ester-bond mutations into these protein systems are discussed.