Publications [#232662] of Michael C. Fitzgerald

Journal Articles

1. Wang, MZ; Fitzgerald, MC, A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides., Analytical chemistry, vol. 73 no. 3 (February, 2001), pp. 625-631, ISSN 0003-2700 [11217772], [doi]
   (last updated on 2026/01/15)

   Abstract:
   Here we report on the application of a solid-solid (SS) sample preparation protocol for the MALDI analysis of peptides and multicomponent peptide mixtures. Our results with a series of model peptides indicate that a SS MALDI sample preparation protocol is useful for the analysis of peptides in the 1-3 kDa mass range. MALDI mass spectra recorded for peptides in this size range using a SS sample preparation were of a quality comparable to spectra recorded using a conventional dried-droplet (DD) sample preparation. Our results with several model peptide mixtures indicate that one advantage of a SS sample preparation protocol for the MALDI analysis of peptides is that it can significantly reduce signal suppression effects in multicomponent mixtures. MALDI results obtained using a SS sample preparation protocol are also more reproducible than results obtained using a conventional DD sample preparation protocol.