Publications [#160650] of Virginia B Kraus

Papers Published

1. DJ Quintana, P Garnero, JL Huebner, N Charni-Ben Tabassi, VB Kraus, PIIANP and HELIXII diurnal variation., Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, England, vol. 16 no. 10 (October, 2008), pp. 1192-5, ISSN 1522-9653
   (last updated on 2009/08/04)

   Abstract:
   OBJECTIVE: Serum levels of procollagen type IIA N-terminal propeptide (sPIIANP) and type-II collagen helical peptide (sHELIXII) biomarkers were evaluated for variation diurnally and with physical activity and food in participants with osteoarthritis (OA) of the knee. METHODS: Forty participants with OA of at least one knee were admitted overnight to the General Clinical Research Center for serial serum sampling. Samples were obtained on the evening (6-8 pm) of Day 1 (T3, n=40); prior to rising (8 am) from bed (T0, n=40); 1 h after rising (9 am) without food consumption (T1a, n=20); 1-2 h after rising (9-10 am) with food consumption (T1, n=40); and additionally at noon, 4 h after rising (T2, n=20). sPIIANP and sHELIXII were measured by enzyme-linked immunosorbent assay. Results were analyzed using non-parametric Freidman's test with Dunn's post-hoc multiple comparison test. RESULTS: Normalized mean concentrations for sPIIANP and sHELIXII increased significantly from T0 to T1 (P<0.05). CONCLUSIONS: This is the first study to demonstrate diurnal variation of these collagen-II biomarkers in individuals with knee OA. These results suggest that serum sampling for these markers should be standardized for purposes of clinical trials.

   Keywords:
   Adult • Aged • Aged, 80 and over • Biological Markers • Cartilage, Articular • Circadian Rhythm • Cohort Studies • Collagen Type II • Female • Humans • Male • Middle Aged • Osteoarthritis, Knee • Peptide Fragments • Statistics as Topic • blood • metabolism • metabolism* • pathology*