Publications [#285324] of Amanda Hargrove

Journal Articles

1. Raskatov, JA; Nickols, NG; Hargrove, AE; Marinov, GK; Wold, B; Dervan, PB, Gene expression changes in a tumor xenograft by a pyrrole-imidazole polyamide., Proceedings of the National Academy of Sciences of the United States of America, vol. 109 no. 40 (October, 2012), pp. 16041-16045, ISSN 0027-8424 [doi]
   (last updated on 2024/12/31)

   Abstract:
   Gene regulation by DNA binding small molecules could have important therapeutic applications. This study reports the investigation of a DNA-binding pyrrole-imidazole polyamide targeted to bind the DNA sequence 5'-WGGWWW-3' with reference to its potency in a subcutaneous xenograft tumor model. The molecule is capable of trafficking to the tumor site following subcutaneous injection and modulates transcription of select genes in vivo. An FITC-labeled analogue of this polyamide can be detected in tumor-derived cells by confocal microscopy. RNA deep sequencing (RNA-seq) of tumor tissue allowed the identification of further affected genes, a representative panel of which was interrogated by quantitative reverse transcription-PCR and correlated with cell culture expression levels.