Publications [#260800] of Ashutosh Chilkoti

Papers Published

1. Piervincenzi, RT; Chilkoti, A, Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor., Biomolecular engineering, vol. 21 no. 1 (January, 2004), pp. 33-42, ISSN 1389-0344 [14715318], [doi]
   (last updated on 2026/01/15)

   Abstract:
   We report here the effect of circular permutation on the structure and function of a model protein tendamistat, a 74 amino acid competitive inhibitor of porcine pancreatic alpha-amylase. The activity and stability of wild type and two permuted tendamistat variants were characterized by measurement of alpha-amylase kinetic and thermodynamic binding parameters and their thermodynamics of unfolding. Our results show that large variations in structure and function can occur upon circularly permuting tendamistat near its active site that are not obvious, a priori, from the structure of the native protein and we propose a structural thermodynamic explanation of the experimental observations.