Publications [#374612] of Ashutosh Chilkoti

Papers Published

1. Sethi, V; Cohen-Gerassi, D; Meir, S; Ney, M; Shmidov, Y; Koren, G; Adler-Abramovich, L; Chilkoti, A; Beck, R, Modulating hierarchical self-assembly in thermoresponsive intrinsically disordered proteins through high-temperature incubation time., Scientific reports, vol. 13 no. 1 (December, 2023), pp. 21688 [doi]
   (last updated on 2026/01/15)

   Abstract:
   The cornerstone of structural biology is the unique relationship between protein sequence and the 3D structure at equilibrium. Although intrinsically disordered proteins (IDPs) do not fold into a specific 3D structure, breaking this paradigm, some IDPs exhibit large-scale organization, such as liquid-liquid phase separation. In such cases, the structural plasticity has the potential to form numerous self-assembled structures out of thermal equilibrium. Here, we report that high-temperature incubation time is a defining parameter for micro and nanoscale self-assembly of resilin-like IDPs. Interestingly, high-resolution scanning electron microscopy micrographs reveal that an extended incubation time leads to the formation of micron-size rods and ellipsoids that depend on the amino acid sequence. More surprisingly, a prolonged incubation time also induces amino acid composition-dependent formation of short-range nanoscale order, such as periodic lamellar nanostructures. We, therefore, suggest that regulating the period of high-temperature incubation, in the one-phase regime, can serve as a unique method of controlling the hierarchical self-assembly mechanism of structurally disordered proteins.