Publications [#178159] of Gayathri R Devi

Papers Published

1. GR Devi, Delivery of phosphorodiamidate morpholino antisense oligomers in cancer cells., Methods in molecular biology (Clifton, N.J.), vol. 542 (2009), pp. 351-61, ISSN 1064-3745 [doi]
   (last updated on 2013/05/16)

   Abstract:
   Phosphorodiamidate morpholino oligomers (PMO), which have a neutral chemistry, are extensively being used as tools for selective inhibition of gene expression in cell culture models and are currently in human clinical trials. PMO oligomers possess a unique structure, in which the deoxyribose moiety of DNA is replaced with a six-membered morpholine ring and the charged phosphodiester internucleoside linkages are replaced with neutral phosphorodiamidate linkages. PMO internalization in uptake-permissive cells has been observed to be specific, saturable, and energy-dependent, suggesting a receptor-mediated uptake mechanism. Understanding PMO transport should facilitate the design of more effective synthetic antisense oligomers as therapeutic agents.

   Keywords:
   Cell Line, Tumor • Gene Transfer Techniques* • Humans • Morpholines • Morpholinos • Neoplasm Proteins • Neoplasms • metabolism • pharmacology* • therapy*