Publications [#99281] of Gayathri R Devi

Papers Published

1. GR Devi, CC Sprenger, SR Plymate, RG Rosenfeld, Insulin-like growth factor binding protein-3 induces early apoptosis in malignant prostate cancer cells and inhibits tumor formation in vivo., The Prostate, vol. 51 no. 2 (May, 2002), pp. 141-52, ISSN 0270-4137 [doi]
   (last updated on 2013/05/16)

   Abstract:
   BACKGROUND: Insulin-like growth factor binding protein (IGFBP-3) levels are significantly reduced in malignant prostate epithelial cells. In this study, we evaluated the role of endogenous IGFBP-3 on prostate cancer cell growth and tumorigenesis. METHODS: IGFBP-3 was re-expressed by stable transfection of human IGFBP-3 cDNA in a model of human prostate cancer, M12, a malignant subline in which IGFBP-3 levels are undetectable in comparison to the parent epithelial cell, P69. Effect of IGFBP-3 re-expression (M12-BP-3) on growth kinetics, morphology, propensity to apoptosis, and in vivo tumor formation were studied. RESULTS: M12-BP-3 cells secreted IGFBP-3 and growth arrested at a cell density that was threefold lower than control cells and this was associated with marked alteration in cell morphology. Control cells when grown in conditioned media secreted by M12-BP-3 also showed altered morphology compared to when cultured in IGFBP-3-immunodepleted conditioned media. The M12-BP-3 clones showed altered mitochondrial membrane potential, increased PARP cleavage, increase in sub-G1 peak, decreased levels of neuron specific enolase, and decreased tumor formation in athymic, nude mice. CONCLUSIONS: These data suggest that IGFBP-3 induces early apoptosis and has potential tumor suppressive effect in prostate cancer. Prostate 51: 141-152, 2002.

   Keywords:
   Apoptosis • Blotting, Western • Cell Division • Cell Transformation, Neoplastic* • Culture Media • DNA, Complementary • DNA, Neoplasm • Electrophoresis, Polyacrylamide Gel • Epithelial Cells • Gene Expression Regulation, Neoplastic* • Humans • Immunohistochemistry • Insulin-Like Growth Factor Binding Protein 3 • Male • Prostatic Neoplasms • Tumor Cells, Cultured • biosynthesis* • drug effects* • genetics • pathology* • pharmacology* • physiology