Publications [#168910] of Mark W. Dewhirst

Papers Published

1. B Yan, H Wang, D Xie, N Wakamatsu, MS Anscher, MW Dewhirst, RE Mitchel, BJ Chen, CY Li, Increased skin carcinogenesis in caspase-activated DNase knockout mice., Carcinogenesis, England, vol. 30 no. 10 (October, 2009), pp. 1776-80, ISSN 1460-2180
   (last updated on 2009/12/31)

   Abstract:
   Caspase-activated DNase (CAD), also called DNA fragmentation factor (DFF), is the enzyme responsible for DNA fragmentation during apoptosis, a hallmark of programmed cell death. CAD/DFF has been shown to suppress radiation-induced carcinogenesis by preventing genomic instability in cells. In this study, we have investigated the role of CAD in chemical carcinogenesis using CAD-null mice and two-stage model of skin carcinogenesis. After topical treatment of mouse skin with dimethylbenz[a]anthracene (DMBA) as an initiator and 12-O-tetradecanoylphorbol-13-acetate (TPA) as a promoting agent, there was a 4-fold increase in the number of papillomas per mouse and 50.8% increase in the incidence of papilloma formation in the CAD knockout mice compared with wild-type littermates. The papillomas in CAD-null mice grew faster and reached larger sizes. These data indicate that loss of CAD function enhances tumorigenesis induced by a chemical carcinogen in the DMBA/TPA two-stage model of skin carcinogenesis in mice.

   Keywords:
   9,10-Dimethyl-1,2-benzanthracene • Animals • Apoptosis • Conserved Sequence • Crosses, Genetic • DNA • DNA Fragmentation • DNA Primers • Deoxyribonucleases • Humans • Mice • Mice, Inbred C57BL • Mice, Knockout • Mice, Transgenic • Neoplasm Staging • Nucleosomes • Skin Neoplasms • deficiency* • drug effects • genetics • genetics* • isolation & purification • pathology • pharmacology