Publications [#234224] of Eric J. Toone

Journal Articles

1. Shelton, MC; Toone, EJ, Differential dye-ligand chromatography as a general purification protocol for 2-keto-3-deoxy-6-phosphogluconate aldolases, Tetrahedron Asymmetry, vol. 6 no. 1 (January, 1995), pp. 207-211, Elsevier BV, ISSN 0957-4166 [doi]
   (last updated on 2026/01/13)

   Abstract:
   2-Keto-3-deoxy-6-phosphogluconate aldolases (KDPG aldolases, EC 4.1.2.14) from four sources, Esherichia coli, Pseudomonas putida, Pseudomonas saccharophila, and Zymononas mobilis, were purified from 17- to 48- fold by differential dye-ligand chromatography (DD-L chromatography). This technique, using Procion Navy H-ER (150 grain) as a negative column and Procion Yellow MX-GR as a positive column supported on Sepharose CL-4B, represents a general methodology for the rapid, facile purification of KDPG aldolases from disparate microbial sources. © 1994.