Publications [#60896] of George A. Truskey

Papers Published

1. Chan, B.P. and Reichert, W.M. and Truskey, G.A., Effect of streptavidin-biotin on endothelial vasoregulation and leukocyte adhesion, Biomaterials (UK), vol. 25 no. 18 (2004), pp. 3951 - 61 [077]
   (last updated on 2007/04/12)

   Abstract:
   The current study examines whether the adhesion promoting arginine-glycine-aspartate-streptavidin mutant (RGD-SA) also affects two important endothelial cell (EC) functions in vitro: vasoregulation and leukocyte adhesion. EC adherent to surfaces via fibronectin (Fn) or Fn plus RGD-SA were subjected to laminar shear flow and media samples were collected over a period of 4 h to measure the concentration of nitric oxide (NO), prostacyclin (PGI₂), and endothelin-1 (ET-I). Western blot analysis was used to quantify the levels of endothelial-derived nitric oxide synthase (eNOS) and cyclooxygenase II (COX II). In a separate set of experiments, fluorescent polymorphonuclear leukocyte (PMN) adhesion to EC was quantified for EC with and without exposure to flow preconditioning. When cell adhesion was supplemented with the SA-biotin system, flow-induced production of NO and PGI₂ increased significantly relative to cells adherent on Fn alone. Previous exposure of EC to shear flow also significantly decreased PMN attachment to SA-biotin supplemented EC, but only after 2 h of exposure to shear flow. The observed decrease in PMN-EC adhesion was negated by N^G-nitro-L-arginine methyl ester (L-NAME), an antagonist of NO synthesis, but not by indomethacin, an inhibitor to PGI₂ synthesis, indicating the induced effect of PMN-EC interaction is primarily NO-dependent. Results from this study suggest that the use of SA-biotin to supplement EC adhesion encourages vasodilation and PMN adhesion in vitro under physiological shear-stress conditions. We postulate that the presence of SA-biotin more efficiently transmits the shear-stress signal and amplifies the downstream events including the NO and PGI₂ release and leukocyte-EC inhibition. These results may have ramifications for reducing thrombus-induced vascular graft failure

   Keywords:
   adhesion;biomedical materials;blood;cellular biophysics;haemorheology;laminar flow;molecular biophysics;nitrogen compounds;shear flow;