Publications [#60932] of George A. Truskey

Papers Published

1. Burmeister, J.S. and Vrany, J.D. and Reichert, W.M. and Truskey, G.A., Effect of fibronectin amount and conformation on the strength of endothelial cell adhesion to HEMA/EMA copolymers, Journal of Biomedical Materials Research, vol. 30 no. 1 (1996), pp. 13 - 22 [(SICI)1097-4636(199601)30:1<13::AID-JBM3>3.0.CO;2-U]
   (last updated on 2007/04/12)

   Abstract:
   The effect of substrate surface hydrophobicity on fibronectin (Fn) adsorption and endothelial cell adhesion strength was studied. Bovine aortic endothelial cells (BAEC) were plated for 2 h with and without preadsorbed Fn on slides coated with homopolymers and copolymers of hydrophilic polyhydroxyethylmethacrylate (polyHEMA) and hydrophobic polyethylmethacrylate (polyEMA). The amount of adsorbed Fn was determined using ¹²⁵I-labeled Fn. BAEC were cultured in serum-free medium for 2 h and subjected to a brief exposure of laminar flow in a variable-height flow chamber that provided a range of shear stresses of 15-185 dynes/cm². The critical shear stress to detach 50% of the cells increased with increasing EMA content to a maximum at 20% HEMA/80% EMA copolymer irrespective of the presence of preadsorbed Fn. However, the critical force increased even though there were similar amounts of Fn adsorbed on all substrates. Thus, the force to detach cells did not increase solely with increasing amounts of adsorbed Fn.

   Keywords:
   Adhesion;Polyacrylates;Copolymers;Surface properties;