Publications [#279948] of Farshid Guilak

Papers Published

1. Estes, BT; Guilak, F, Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells., Methods in molecular biology (Clifton, N.J.), vol. 702 (2011), pp. 201-217 [21082404], [doi]
   (last updated on 2019/06/30)

   Abstract:
   Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.