Publications [#279998] of Farshid Guilak

Papers Published

1. Guilak, F, Cytoskeletal regulation of nuclear deformation and intracellular prestress in chondrocytes, American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, vol. 28 (1994), pp. 213-214, Chicago, IL, USA
   (last updated on 2019/06/30)

   Abstract:
   Changes in chondrocyte shape and volume are believed to play a role in the mechanical signal transduction pathway of articular cartilage. To examine how chondrocyte deformation may be transduced to an intracellular signal, confocal microscopy was used to perform three-dimensional morphometric analyses of chondrocyte nuclei during applied deformation of the cartilage matrix. Uniaxial compression of the matrix resulted in a significant decrease in nuclear height in the direction of compression, concomitant with decreases in overall chondrocyte height and volume. Disruption of the actin cytoskeleton resulted in a decrease in nuclear size and prevented nuclear deformation during matrix loading. These findings suggest that the chondrocyte nucleus exists in a state of tensile prestress, and that the actin cytoskeleton plays an important role in regulating nuclear deformation.

   Keywords:
   Deformation;Microscopic examination;Three dimensional;Cartilage;Proteins;Morphology;Physiology;Metabolites;