Publications [#113092] of Harold P. Erickson

Papers Published

1. HR Brown, HP Erickson, Assembly of proteolytically cleaved tubulin., Archives of biochemistry and biophysics, UNITED STATES, vol. 220 no. 1 (January, 1983), pp. 46-51, ISSN 0003-9861
   (last updated on 2009/02/12)

   Abstract:
   Conditions have been found for limited proteolysis of purified tubulin, in which 70-90% of the molecules are cleaved at one or two sites. Thermolysin and chymotrypsin cleave the alpha and beta subunits, respectively, at single sites. Trypsin cleaves the alpha subunit at two sites. The chymotrypsin site and one of the trypsin sites are apparently inaccessible on assembled microtubules. The different samples of proteolyzed tubulin were all fully competent to assemble in a buffer containing 1 M sodium glutamate. In another buffer (50 mM morpholinoethanesulfonic acid, 3.4 M glycerol) tubulin digested by thermolysin assembled as well as native tubulin, but samples digested by chymotrypsin or trypsin would not assemble even at high protein concentrations.

   Keywords:
   Binding Sites • Buffers • Chemistry • Peptide Hydrolases* • Protein Binding • Tubulin*