Publications [#113117] of Harold P. Erickson

Papers Published

1. KC Ingham, SA Brew, HP Erickson, Localization of a cryptic binding site for tenascin on fibronectin., The Journal of biological chemistry, United States, vol. 279 no. 27 (July, 2004), pp. 28132-5, ISSN 0021-9258
   (last updated on 2009/02/12)

   Abstract:
   Fibronectin and tenascin are large extracellular matrix proteins that interact with each other and with integrin receptors to regulate cell growth and movement. They are both modular proteins composed of independently folded domains (modules) that are arranged in linear fashion. Fibronectin is a covalent dimer and tenascin is a hexamer. The site on tenascin to which fibronectin binds has been localized to type III modules 3-5. In this study we use surface plasmon resonance to examine the interaction between various fragments of fibronectin and tenascin to further characterize and localize the binding sites. We found that tenascin fragments that contain type III modules 3-5 bind primarily to the N-terminal 29-kDa hep-1/fib-1 domain, which contains the first five type I modules of fibronectin. The dissociation constant, K(d), is approximately 1 microm. The binding site on fibronectin appears to be cryptic in the whole molecule in solution but is exposed on the proteolytic fragments and probably when fibronectin is in the extended conformation.

   Keywords:
   Animals • Binding Sites • Cell Division • Dimerization • Extracellular Matrix • Fibronectins • Kinetics • Models, Genetic • Protein Binding • Protein Conformation • Protein Folding • Protein Structure, Tertiary • Spectrophotometry • Surface Plasmon Resonance • Swine • Temperature • Tenascin • Time Factors • chemistry* • metabolism