Publications [#113187] of Harold P. Erickson

Papers Published

1. DE Staunton, ML Dustin, HP Erickson, TA Springer, The arrangement of the immunoglobulin-like domains of ICAM-1 and the binding sites for LFA-1 and rhinovirus., Cell, UNITED STATES, vol. 61 no. 2 (April, 1990), pp. 243-54, ISSN 0092-8674
   (last updated on 2009/02/12)

   Abstract:
   Intercellular adhesion molecule 1 (ICAM-1, CD54) binds to the integrin LFA-1 (CD11a/CD18), promoting cell adhesion in immune and inflammatory reactions. ICAM-1 is also subverted as a receptor by the major group of rhinoviruses. Electron micrographs show that ICAM-1 is a bent rod, 18.7 nm long, suggesting a model in which the five immunoglobulin-like domains are oriented head to tail at a small angle to the rod axis. ICAM-1 sequences important to binding LFA-1, rhinovirus, and four monoclonal antibodies were identified through the characterization of chimeric ICAM-1 molecules and mutants. The amino-terminal two immunoglobulin-like domains of ICAM-1 appear to interact conformationally. Domain 1 of ICAM-1 contains the primary site of contact for both LFA-1 and rhinovirus; the presence of domains 3-5 markedly affects the accessibility of the binding site for rhinovirus and less so for LFA-1. The binding sites appear to be distinct but overlapping; rhinovirus binding also differs from LFA-1 binding in its lack of divalent cation dependence. Our analysis suggests that rhinoviruses mimic LFA-1 in binding to the most membrane-distal, and thus most accessible, site of ICAM-1.

   Keywords:
   Amino Acid Sequence • Animals • Antigens, CD • Antigens, Differentiation • Cell Adhesion Molecules • Cell Line • Chimera • Chromosome Deletion • Humans • Immunoglobulins • Intercellular Adhesion Molecule-1 • Kinetics • Lymphocyte Function-Associated Antigen-1 • Membrane Glycoproteins • Mice • Models, Molecular • Molecular Sequence Data • Mutation • Oligonucleotide Probes • Protein Binding • Protein Conformation • Receptors, Leukocyte-Adhesion • Receptors, Virus • Rhinovirus • genetics • genetics* • immunology • immunology* • metabolism • metabolism* • physiology*