Publications [#80879] of Russell P. Hall III

Papers Published

1. RP Hall, JC Murray, MM McCord, MJ Rico, RD Streilein, Rabbits immunized with a peptide encoded for by the 230-kD bullous pemphigoid antigen cDNA develop an enhanced inflammatory response to UVB irradiation: a potential animal model for bullous pemphigoid., The Journal of investigative dermatology, UNITED STATES, vol. 101 no. 1 (July, 1993), pp. 9-14, ISSN 0022-202X
   (last updated on 2007/03/27)

   Abstract:
   Previous attempts to develop an animal model of bullous pemphigoid (BP) have failed to result in inflammatory disease in the skin. P1-2 is an 18-amino acid peptide encoded for by the 230-kD BP antigen cDNA that has been shown to contain an epitope recognized by circulating antibodies from patients with BP. The purpose of this study was to determine if ultraviolet B irradiation of rabbits after immunization with the P1-2 peptide would result in an enhanced inflammatory response in the skin to that injury. Three rabbits were immunized with either P1-2 or a control peptide. All rabbits immunized with P1-2, and none of the control rabbits, developed antibodies against P1-2 that bound in vitro to both human and rabbit skin in a linear pattern at the basement membrane zone. Immunized rabbits were irradiated on the flank with ultraviolet light. Rabbits immunized with P1-2 developed an enhanced inflammatory reaction to ultraviolet B irradiation leading to epidermal necrosis and sloughing of some sites in 6-9 d. Control rabbits showed only mild erythema without sloughing, which healed in 4-6 d. Histology in the P1-2 immunized rabbits at 24 h revealed an inflammatory infiltrate of neutrophils at the dermal-epidermal junction, whereas control rabbits showed only mild edema and a sparse inflammatory infiltrate. All the rabbits immunized with P1-2 had linear deposits of immunoglobulin G and C3 at the basement membrane zone of healed skin compared to none of the controls. These findings demonstrate that antibodies against a synthetic peptide encoded by the BP antigen 1 sequence can lead to an enhanced inflammatory response after epithelial injury in rabbit skin.

   Keywords:
   Animals • Autoantigens • Carrier Proteins* • Collagen* • Cytoskeletal Proteins* • DNA • Disease Models, Animal • Female • Fluorescent Antibody Technique • Immunization* • Nerve Tissue Proteins* • Non-Fibrillar Collagens* • Pemphigoid, Bullous • Peptides • Rabbits • Radiation Injuries, Experimental • Skin • Ultraviolet Rays* • genetics • immunology • immunology* • pathology • radiation effects