Publications [#80890] of Russell P. Hall III

Papers Published

1. MJ Rico, LD White, S Bartow, RP Hall, Detection of cross-reactive idiotypes in the serum of patients with bullous pemphigoid., The Journal of investigative dermatology, UNITED STATES, vol. 96 no. 6 (June, 1991), pp. 809-14, ISSN 0022-202X
   (last updated on 2007/03/27)

   Abstract:
   To further characterize the circulating antibasement membrane zone (antiBMZ) antibodies present in the sera of patients with bullous pemphigoid (BP), we have generated a mouse monoclonal anti-idiotypic antibody (antiId 3-17) specific for an IgG antiBMZ antibody. AntiId 3-17 is specific for an idiotype expressed on antiBMZ IgG in the serum of a patient with BP, and not expressed on pooled normal human IgG or IgG from patients with other autoimmune skin diseases. AntiId 3-17 binds to non-reduced, but not reduced, antiBMZ IgG on immunoblot, suggesting that the idiotype is composed of a conformational epitope expressed on native antibody. By a competitive inhibition ELISA, antiId 3-17 detects a cross-reactive idiotype (CRI) expressed in 18 of 50 (36%) of the sera of patients with BP, but in the sera of only 1 of 50 (2%) normal blood bank controls (p less than 0.001, Fisher's exact test) and 1 of 12 (8%) patients with pemphigus (p = 0.005). Thus, antiId 3-17 recognizes a public idiotype on a native antiBMZ antibody from a patient with BP, which is expressed in the sera of 36% of the unrelated patients with BP studied.

   Keywords:
   Antibodies, Anti-Idiotypic • Antibodies, Monoclonal • Basement Membrane • Chromatography, Affinity • Cross Reactions • Enzyme-Linked Immunosorbent Assay • Humans • Immunoblotting • Immunoglobulin G • Immunoglobulin Idiotypes • Pemphigoid, Bullous • analysis • immunology • immunology* • methods