Publications [#67729] of Richard B. Fair

Papers Published

1. Srinivasan, Vijay and Pamula, Vamsee K. and Fair, Richard B., Droplet-based microfluidic lab-on-a-chip for glucose detection, Analytica Chimica Acta, vol. 507 no. 1 (2004), pp. 145 - 150 [030]
   (last updated on 2007/04/17)

   Abstract:
   A microfluidic lab-on-a-chip (LoC) platform for in vitro measurement of glucose for clinical diagnostic applications is presented in this paper. The LoC uses a discrete droplet format in contrast to conventional continuous flow microfluidic systems. The droplets act as solution-phase reaction chambers and are manipulated using the electrowetting effect. Glucose is measured using a colorimetric enzyme-kinetic method based on Trinder's reaction. The color change is detected using an absorbance measurement system consisting of a light emitting diode and a photodiode. The linear range of the assay is 9-100mg/dl using a sample dilution factor of 2 and 15-300mg/dl using a sample dilution factor of 3. The results obtained on the electrowetting system compare favorably with conventional measurements done on a spectrophotometer, indicating that there is no change in enzyme activity under electrowetting conditions. © 2004 Elsevier B.V. All rights reserved.

   Keywords:
   Drop formation;Enzyme kinetics;Light emitting diodes;Color;Spectrophotometers;