Publications [#228453] of Steven B. Haase

Papers Published

1. Haase, SB, Cell cycle analysis of budding yeast using SYTOX Green., Current protocols in cytometry, vol. Chapter 7 (November, 2004), pp. Unit-7.23 [18770800], [doi]
   (last updated on 2026/01/14)

   Abstract:
   Flow cytometric determination of DNA content is a standard tool for cell cycle analysis in the budding yeast, Saccharomyces cerevisiae. Using SYTOX Green instead of propidium iodide (PI) in a standard ethanol fixation protocol increases the accuracy and reproducibility of cell cycle analyses. SYTOX-stained cells exhibit improved coefficients of variation (CVs), a better correlation between DNA content and fluorescence, and decreased sensitivity to variances in experimental conditions when compared with PI-stained cells. The SYTOX Green protocol provides researchers with a more accurate and reliable alternative to standard PI-based protocols for the analysis of the budding yeast cell cycle.