Publications [#262913] of Tuan Vo-Dinh

Papers Published

1. Vo-Dinh, T; Uziel, M, Laser-induced room-temperature phosphorescence detection of benzo[a]pyrene-DNA adducts., Analytical chemistry, vol. 59 no. 8 (April, 1987), pp. 1093-1095, ISSN 0003-2700 [3592221], [doi]
   (last updated on 2026/01/18)

   Abstract:
   The room-temperature phosphorescence (RTP) spectrum of benzo[a]pyrene-r-7,t-8,9,10-tetrahydrotetrol (BP-tetrol) has been measured using laser excitation. The BP-tetrol was obtained by acid hydrolysis of the r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE)-DNA adducts. BPDE is the ultimate carcinogenic metabolite of benzo[a]pyrene (BP). The BP-tetrol sample was measured on a filter paper substrate pretreated with a heavy-atom salt, thallium acetate, used to increase the phosphorescence signal of BP-tetrol. The detection limit of BPDE in in vitro modified BPDE-DNA was about 15 fmol. The results indicate the RTP would be useful as a simple and practical screening tool for monitoring BPDE-DNA adducts and related BP metabolites in biological samples

   Keywords:
   DNA;organic compounds;phosphorescence;