Publications [#151618] of Walter T Lee

Papers Published

1. WT Lee, K Shimizu, H Kuriyama, H Tanaka, J Kjaergaard, S Shu, Tumor-dendritic cell fusion as a basis for cancer immunotherapy., Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, vol. 132 no. 5 (May, 2005), pp. 755-64, ISSN 0194-5998 [doi]
   (last updated on 2013/05/16)

   Abstract:
   OBJECTIVE: To establish the basis for use of allogeneic dendritic-tumor fusion cells. METHODS: Fusion cells were created by electrofusion. We used 2 allogeneic murine tumor lines (D5 and 4T1) that were virally transduced to express the antigen (beta-galactosidase) as a surrogate tumor marker. RESULTS: Cross-immunization was achieved with irradiated allogenic tumor cells. Successful electrofusion of dendritic cells and tumor cells was confirmed by using fluorescence-activated cell sorting and cytospin. Significant responses were shown in immunized mice against tumor challenge and established 3-day pulmonary metastasis with fusion cells. CONCLUSIONS: Allogeneic tumor sharing a common tumor antigen can immunize against syngeneic tumor challenge. Fusion cells showed successful immunization against tumor challenge and showed regression of 3-day established pulmonary metastasis. CONCLUSIONS: These preclinical studies provide evidence that an allogenic tumor-dendritic cell fusion vaccine is a valid approach for head and neck cancer immunotherapy.

   Keywords:
   Animals • Cell Fusion • Dendritic Cells • Electric Stimulation • Flow Cytometry • Hybrid Cells • Immunotherapy • Lac Operon • Lung Neoplasms • Mice • Mice, Inbred BALB C • Mice, Inbred Strains • Neoplastic Stem Cells • Spleen • Vaccination • beta-Galactosidase • cytology • immunology • immunology* • methods* • physiology • secondary • therapy*