Publications [#178647] of Walter T Lee

Papers Published

1. EI Cho, C Tan, GK Koski, PA Cohen, S Shu, WT Lee, Toll-like receptor agonists as third signals for dendritic cell-tumor fusion vaccines., Head & neck, vol. 32 no. 6 (June, 2010), pp. 700-7, ISSN 1097-0347 [doi]
   (last updated on 2013/05/16)

   Abstract:
   BACKGROUND: The aim of the present study was to evaluate the therapeutic efficacy of dendritic cell (DC)-tumor fusion hybrids with Toll-like receptor (TLR) agonists. METHODS: DC-tumor fusion hybrids were generated by electrofusion and injected into the inguinal lymph nodes of C57BL/6 mice with 3-day established pulmonary metastases. Paired TLR agonists polyinosine:polycytadilic acid [poly(I:C)] and cytosine-phosphate-guanine (CpG) were then injected intraperitoneally. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate interleukin (IL)-12 production from the DC-tumor fusion hybrids in vitro. RESULTS: Fusion + TLR agonists (60 metastases) had significantly fewer metastases than did the untreated control (262 metastases, p = .0001) and fusion alone (150 metastases, p = .02). ELISA showed that the DC-tumor fusion hybrids yielded 90 pg of IL-12 after TLR stimulation compared with 1610 pg from dendritic cells alone. CONCLUSIONS: CpG and poly(I:C) administered as a third signal with fusion hybrids as described significantly reduce melanoma metastasis compared with fusion hybrids alone. Fusion hybrids do not appear to be a significant source for IL-12 secretion.

   Keywords:
   Adjuvants, Immunologic • Animals • Cell Fusion • Dendritic Cells • Electric Stimulation • Enzyme-Linked Immunosorbent Assay • Female • Hybrid Cells • Immunotherapy • Interleukin-12 • Lung Neoplasms • Melanoma, Experimental • Mice • Mice, Inbred C57BL • Recombinant Fusion Proteins • Specific Pathogen-Free Organisms • Toll-Like Receptors • Vaccines, Synthetic • agonists* • immunology • immunology* • metabolism • methods* • secondary • therapy*