Publications [#65212] of Michael R. Zalutsky

Papers Published

1. Walicka, M.A. and Vaidyanathan, G. and Zalutsky, M.R. and Adelstein, S.J. and Kassis, A.I., Survival and DNA damage in Chinese hamster V79 cells exposed to alpha particles emitted by DNA-incorporated astatine-211, Radiat. Res. (USA), vol. 150 no. 3 , pp. 263 - 8
   (last updated on 2007/04/15)

   Abstract:
   Asynchronous Chinese hamster V79 lung fibroblasts were incubated at 37°C for 30 min with the thymidine analog 5-[²¹¹At]astato-2'-deoxyuridine (²¹¹AtdU, exposure from DNA-incorporated activity) or with [²¹¹At]astatide (²¹¹At^-, exposure from extracellular activity), and DNA-incorporated activity was determined. The ²¹¹AtdU content in cellular DNA increased as a function of extracellular concentration. Incorporation of ²¹¹At^- was less than 1% of that of ²¹¹AtdU, after exposure, cells were frozen in the presence of 10% DMSO. One month later, survival was determined by the colony-forming assay, and DNA double-strand breaks (DSBs) were measured by the neutral elution method (pH 9.6). The survival curve for ²¹¹AtdU was biphasic (D₃₇=2.8 decays per cell), reflecting killing of ²¹¹At-DNA-labeled cells and of unlabeled cells irradiated by ²¹¹At in neighboring labeled cells. The toxicity of ²¹¹At^- decaying outside the cell (30-min exposure) was negligible, analysis of the survival curve produced a D₀ of 1.3 decays/cell for ²¹¹At-labeled cells. The yield of DSBs from the decay of DNA-incorporated ²¹¹At was compared with that from DNA-incorporated ¹²⁵I. Each decay of ²¹¹At produced at least 10 times the number of DSBs as that obtained per ¹²⁵I decay. The extreme radiotoxicity of DNA-incorporated ²¹¹AtdU seems to be associated with considerable damage to the mammalian cell genome

   Keywords:
   alpha-particle effects;astatine;biological effects of ionising particles;biomolecular effects of radiation;cellular effects of radiation;DNA;lung;