Publications [#65232] of Michael R. Zalutsky

Papers Published

1. Garg, Pradeep K. and Archer, Gerald E. Jr. and Bigner, Darell D. and Zalutsky, Michael R., Synthesis of radioiodinated N-succinimidyl iodobenzoate. Optimization for use in antibody labelling, Applied Radiation and Isotopes, vol. 40 no. 6 (1989), pp. 485 - 490 [0883-2889(89)90131-7]
   (last updated on 2007/04/15)

   Abstract:
   N-succinimidyl-3-(tri-n-butylstannyl)benzoate (m-BuATE), N-Succinimidyl-3-(tri-methylstannyl)benzoate (m-MeATE) and N-succinimidyl-4-(tri-n-butylstannyl)benzoate (p-BuATE) were synthesized and radio-iodinated using either N-chlorosuccinimide (NCS) or t-butylhydroperoxide (TBHP) as the oxidant. Radiohalogenation of m-MeATE proceeded more rapidly than m-BuATE. NCS was the more efficient oxidant at reaction times < 15 min; use both TBHP and NCS resulted in nearly quantitative yields after 15 min when m-MeATE was used. Using NCS, achieving optimal antibody coupling and specific binding required purification of the active ester by HPLC; in contrast, with TBHP, only Sep Pak purification was needed.

   Keywords:
   Radioactive Materials--Tracers;Isotopes;