|
| Publications [#230009] of Zhen-Ming Pei
search www.ncbi.nlm.nih.gov.Papers Published
- Yang, P; Shang, YF; Pei, ZM, Action Mechanism Research of Lanthanons to Slow Vacuolar Ion Channels in Raphanus Satirus L. (Xinlimei) Radish by Patch-Clamp,
Chinese Chemical Letters, vol. 12 no. 9
(September, 2001),
pp. 813-814
(last updated on 2026/01/17)
Abstract: We used whole-vacuolar patch-clamp recording mode to study the action mechanism of La3+ to Slow Vacuolar (SV) channels for the first time. We recorded SV channel currents of Xinlimei (Raphanus satirus L.) vacuolars. The minimum activation potentials of voltage-dependent SV channels lied in 25±5 mV. The increase in cytoplasmic Ca2+ led to enhancement of SV-type currents. It was found that the threshold potential of activation shifted towards more depolarized values whenever cytoplasmic Ca2+ was increased. When 10-10 mol/L free La3+ was added to the bath, SV-type current was suppressed by 60-75%. These data showed La3+ reduced ion permeabilities of Xinlimei root vacuolar membrane.
|