Publications [#62173] of Kam W. Leong

Papers Published

1. Feng, Q. and Jiang, X.S. and Chai, C. and Lim, W.S. and Leong, K.W. and Mao, H.Q., Ex vivo expansion of hematopoietic primitive cells on ECM molecule-immobilized scaffold, Transactions - 7th World Biomaterials Congress (2004), pp. 898 -, Sydney, Australia
   (last updated on 2007/04/13)

   Abstract:
   The ex vivo expansion of hematopoietic stem sells (HSC) on extracellular matrix (ECM) molecule-immobilized scaffold was investigated. The scaffold used was a fibronectin and collagen conjugated polyethylene terephthalate (PET) scaffold. Bone marrow CD34+ cells were cultured in the modified PET scaffolds in a 96-well plate using serum free StemSpam medium supplemented with cytokines. It was observed that the scaffolds surface-conjugated with ECM molecules served as an effective substrate for the expansion of hematopoietic primitive cells. Fibronectin conjugated scaffold was found to exhibit the highest extent of CD34+ cell expansion.

   Keywords:
   Cell immobilization;Bone;Collagen;Polyethylene terephthalates;Transplantation (surgical);Medical problems;Disease control;Cell membranes;Solubility;Suspensions (fluids);pH effects;Crosslinking;