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| Publications [#215322] of Richard T. Di Giulio
Papers Published
- LV Garner, RT Di Giulio, Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos.,
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, vol. 155 no. 4
(May, 2012),
pp. 573-9, ISSN 1532-0456
(last updated on 2013/01/25)
Abstract:
Glutathione transferases (GSTs) are phase II enzymes that detoxify a wide range of toxicants and reactive intermediates. One such class of toxicants is the ubiquitous polycyclic aromatic hydrocarbons (PAHs). Certain PAHs are known to cause developmental cardiac toxicity in fish. Herein, we explored the role of GST pi class 2 (GSTp2) in PAH- and PCB-induced cardiac toxicity in zebrafish (Danio rerio) embryos. We measured expression of GSTp2 in embryos exposed to individual and co-exposures of the PAHs benzo[k]fluoranthene (BkF), benzo[a]pyrene (BaP), and fluoranthene (FL) as well as 3,3',4,4',5-pentachlorobiphenyl (PCB-126). GSTp2 mRNA expression was induced by exposure to BkF, BaP, PCB-126, and BaP+FL and BkF+FL co-exposure. A splice junction morpholino was then used to knockdown GSTp2 in developing zebrafish. GSTp2 knockdown exacerbated the toxicity caused by co-exposures to BkF+FL and BaP+FL. However, GSTp2 knockdown did not affect PCB-126 toxicity. These results further suggest that pi class GSTs serve a protective function against the synergistic toxicity caused by PAHs in developing zebrafish.
Keywords:
Animals • Drug Synergism • Drug Therapy, Combination • Embryo, Nonmammalian • Embryonic Development • Estrogen Antagonists • Gene Expression Regulation, Developmental • Gene Knockdown Techniques • Gene Silencing • Glutathione S-Transferase pi • Heart Defects, Congenital* • Morpholinos • Polychlorinated Biphenyls • Polycyclic Hydrocarbons, Aromatic • Zebrafish • administration & dosage • chemically induced • classification • drug effects • drug effects* • enzymology • genetics • metabolism • metabolism* • physiology • prevention & control • toxicity*
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