Publications [#381249] of Seog Oh

Papers Published

1. Sivalingam, J; SuE, Y; Lim, ZR; Lam, ATL; Lee, AP; Lim, HL; Chen, HY; Tan, HK; Warrier, T; Hang, JW; Nazir, NB; Tan, AHM; Renia, L; Loh, YH; Reuveny, S; Malleret, B; Oh, SKW, A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells., Stem cell reports, vol. 16 no. 1 (January, 2021), pp. 182-197 [doi]
   (last updated on 2026/01/20)

   Abstract:
   Universal red blood cells (RBCs) differentiated from O-negative human induced pluripotent stem cells (hiPSCs) could find applications in transfusion medicine. Given that each transfusion unit of blood requires 2 trillion RBCs, efficient bioprocesses need to be developed for large-scale in vitro generation of RBCs. We have developed a scalable suspension agitation culture platform for differentiating hiPSC-microcarrier aggregates into functional RBCs and have demonstrated scalability of the process starting with 6 well plates and finally demonstrating in 500 mL spinner flasks. Differentiation of the best-performing hiPSCs generated 0.85 billion erythroblasts in 50 mL cultures with cell densities approaching 1.7 × 10⁷ cells/mL. Functional (oxygen binding, hemoglobin characterization, membrane integrity, and fluctuations) and transcriptomics evaluations showed minimal differences between hiPSC-derived and adult-derived RBCs. The scalable agitation suspension culture differentiation process we describe here could find applications in future large-scale production of RBCs in controlled bioreactors.