Publications [#381443] of Seog Oh

Papers Published

1. Chen, S; Choo, ABH; Nai-Dy, W; Heng-Phon, T; Oh, SKW, Knockdown of Oct-4 or Sox-2 attenuates neurogenesis of mouse embryonic stem cells., Stem cells and development, vol. 16 no. 3 (June, 2007), pp. 413-420 [doi]
   (last updated on 2026/01/20)

   Abstract:
   We employed a stromal-derived inducing activity (SDIA) model of neurogenesis to investigate the effects of targeted knockdown of Oct-4 and Sox-2 by short interfering RNAs (siRNAs) in mouse embryonic stem (mES) cells. Quantitative real-time PCR showed 40-90% knockdown of specific transcripts with cognate Oct-4 or Sox-2 siRNA transfection compared to FAM-labeled negative control (FAM) siRNA or mock transfection and was confirmed at the protein level by western blot analyses. Upon differentiation using PA6 SDIA co-cultures, neurogenesis is significantly diminished in Oct-4 or Sox-2-targeted mES cells. It was observed that 45 +/- 12%, 65 +/- 13%, and 90 +/- 8% of the colonies were stained with neuron-specific beta-tubulin III in Oct-4, Sox-2, and FAM siRNA transfected mES cells, respectively, with similar results observed using neural inducing factors collected from the surface of PA6. Together, our results extend observations for a role of Oct-4 in SDIA and implicate a similar role for Sox-2.