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| Publications [#381493] of Seog Oh
Papers Published
- Oh, SKW; Ng, T; See, LH, A fast assay for developing serum free media for cord blood haematopoietic cells,
Biotechnology Techniques, vol. 9 no. 8
(August, 1995),
pp. 577-582 [doi]
(last updated on 2026/01/19)
Abstract: One of the bottlenecks in haematopoietic cell expansion is the lack of a defined serum free media. We describe a fast method of monitoring live haematopoietic cell numbers, using fluorescein diacetate, to facilitate the development of such a media. Several carbohydrates (fructose at 500μg/ml and pyruvate at 300μg/ml) and lipids (cholesterol and lecithin, both at 10μg/ml) were tested and could replace serum in cord blood haematopoietic cell cultures. © 1995 Chapman & Hall.
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