Publications [#386444] of Seog Oh

Papers Published

1. Hwang, S-M; Li, J; Koo, N-Y; Choi, S-Y; Lee, SJ; Oh, S-B; Castro, R; Kim, J-S; Park, K, Role of purinergic receptor in alpha fodrin degradation in Par C5 cells., Journal of dental research, vol. 88 no. 10 (October, 2009), pp. 927-932 [doi]
   (last updated on 2026/01/19)

   Abstract:
   Autoantibodies specific for alpha-fodrin fragments are found in the tissues of persons afflicted with Sjögren's syndrome (SS). However, the mechanism for alpha-fodrin degradation remains elusive. The following experiments utilized Par C5 cells to examine the role of P2X7 receptor (P2X7R) in apoptosis, particularly in the cleavage and release of alpha-fodrin, an apparent SS autoantigen. Five mM ATP stimulation induced apoptotic cell death with a sustained Ca2+ influx, which was mimicked in HEK cells transfected with P2X7R. ATP also induced cleavage of alpha-fodrin mediated by caspase-3 and calpain, releasing alpha-fodrin fragments through membrane blebs. However, both apoptotic cell death and alpha-fodrin cleavage were inhibited in the presence of 300 microM oxidized-ATP (ox-ATP), an irreversible blocker of P2X7R, or in Ca(2+)-free solution. We concluded that P2X7R plays an important role in apoptosis and alpha-fodrin degradation in salivary epithelial cells, providing an important clue elucidating the presence of alpha-fodrin fragments in SS tissues.