Publications [#99806] of Bruce A Sullenger

Papers Published

1. SW Lee, BA Sullenger, Isolation of a nuclease-resistant decoy RNA that selectively blocks autoantibody binding to insulin receptors on human lymphocytes., The Journal of experimental medicine, vol. 184 no. 2 (August, 1996), pp. 315-24, ISSN 0022-1007
   (last updated on 2013/05/16)

   Abstract:
   An RNA containing 2'-amino pyrimidines has been isolated using in vitro selection techniques that specifically and avidly (apparent Kd approximately 30 nM) binds a mouse monoclonal antibody called MA20. This 2'-amino-derivatized RNA is at least 10,000-fold more stable than unmodified RNA in serum, and can act as a decoy and block MA20 binding to its natural antigen, the human insulin receptor, on lymphocytes. Furthermore, this RNA decoy can inhibit MA20-mediated downmodulation of insulin receptor expression on human lymphocytes in culture by up to 90%. Surprisingly, the decoy RNA cross-reacts with autoantibodies from patients with extreme insulin resistance and can inhibit these antiinsulin receptor antibodies from downmodulating insulin receptor expression by up to 80% without impeding insulin binding to its receptor. These results suggest that in vitro-selected decoy RNAs may be able to specifically and selectively block oligoclonal autoimmune responses to self-antigens in patients with autoimmune diseases.

   Keywords:
   Animals • Antibodies, Monoclonal • Autoantibodies • Base Sequence • Binding, Competitive • Down-Regulation • Humans • Insulin Resistance • Lymphocytes • Mice • Molecular Sequence Data • Nucleic Acid Conformation • Protein Binding • RNA • Receptor, Insulin • Ribonucleases • immunology* • metabolism • metabolism*