Publications [#99807] of Bruce A Sullenger

Papers Published

1. JT Jones, SW Lee, BA Sullenger, Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells., Nature medicine, vol. 2 no. 6 (June, 1996), pp. 643-8, ISSN 1078-8956
   (last updated on 2013/05/16)

   Abstract:
   In mammalian cells, genetic instructions are usually revised by RNA splicing before they are translated to proteins. Here we demonstrate that a trans-splicing group I ribozyme can be employed to intentionally modify the sequence of targeted transcripts in tissue culture cells. By analyzing the ribozyme reaction products, we demonstrate that targeted trans-splicing can proceed in murine fibroblasts with high fidelity, providing direct evidence that ribozymes function as anticipated in a therapeutically relevant setting. Trans-splicing is not very specific however, and the ribozyme reacted with and tagged a variety of cellular transcripts with its 3' exon sequence. RNA tagging provides a unique approach to study RNA catalysis in mammalian cells. Such analysis should facilitate the logical development of safe, therapeutic ribozymes that can repair mutant RNAs associated with a variety of inherited diseases.

   Keywords:
   3T3 Cells • Animals • Base Sequence • Cytoplasm • DNA-Directed RNA Polymerases • Lac Operon • Mice • Models, Genetic • Molecular Sequence Data • Plasmids • Polymerase Chain Reaction • RNA • RNA Splicing* • RNA, Bacterial • RNA, Catalytic • Substrate Specificity • Tetrahymena thermophila • Transcription, Genetic* • Transfection • Viral Proteins • chemistry • chemistry* • genetics • genetics* • metabolism • physiology