Publications [#99840] of Bruce A Sullenger

Papers Published

1. TC Lee, BA Sullenger, HF Gallardo, GE Ungers, E Gilboa, Overexpression of RRE-derived sequences inhibits HIV-1 replication in CEM cells., The New biologist, vol. 4 no. 1 (January, 1992), pp. 66-74, ISSN 1043-4674
   (last updated on 2013/05/16)

   Abstract:
   Overexpression of sequences corresponding to the major Rev-binding site in the Rev response element of human immunodeficiency virus type 1 (HIV-1) (RRE decoys) was used to render cells resistant to HIV-1 replication. This was accomplished by the use of a chimeric tRNA-RRE transcription unit in a double-copy murine retroviral vector to express high levels of HIV-1 RRE-containing transcripts in CEM SS cells. Replication of HIV-1 was inhibited more than 90% in cells expressing chimeric tRNA-RRE transcripts, as determined by in situ immunofluorescence analysis and a p24 antigen ELISA test. Analysis of RNA from HIV-1-infected cells suggests that expression of RRE-containing sequences in CEM SS cells inhibits HIV-1 replication by interfering with Rev function, presumably by competing for Rev binding to its physiological target. The use of a subfragment of RRE as decoy RNA reduces the likelihood that essential cellular factors will be sequestered in cells expressing the decoy RNA. Thus, use of RRE-based decoy RNA to inhibit HIV-1 replication may represent a safer alternative to the use of TAR decoy RNA.

   Keywords:
   Base Sequence • Cell Line • Genes, rev • HIV-1 • Humans • Molecular Sequence Data • RNA, Transfer • RNA, Viral • Regulatory Sequences, Nucleic Acid • Virus Replication • genetics* • physiology • physiology*