Publications [#86237] of Anna Mae Diehl

Papers Published

1. A Suzuki, S McCall, SS Choi, JK Sicklick, J Huang, Y Qi, M Zdanowicz, T Camp, YX Li, AM Diehl, Interleukin-15 increases hepatic regenerative activity., Journal of hepatology, England, vol. 45 no. 3 (September, 2006), pp. 410-8, ISSN 0168-8278
   (last updated on 2007/07/23)

   Abstract:
   BACKGROUND/AIMS: Interleukin-15 (IL-15) is expressed in many organs. It generally inhibits apoptosis and increases cellular proliferation and differentiation. However, IL-15's roles in liver are unknown. We aimed to determine if IL-15 influences hepatic integrity and regenerative activity. METHODS: Expression of IL-15 and its receptors was evaluated in several liver injury models, primary hepatocytes, and two liver cell lines. Effects of IL-15 on viability, proliferation, and apoptosis were assessed in cultured liver cells, and also in the livers of healthy mice. RESULTS: IL-15 and its receptors are expressed constitutively in healthy livers, and ligand expression is induced in injured livers. Cultured primary hepatocytes and liver cell lines express IL-15 and its receptors. Administration of IL-15 has minimal effects on cultured liver cells, but significantly up-regulates oval cell accumulation, cyclin mRNA expression, and mature hepatocyte replication in healthy mice. These effects are associated with focal hepatic inflammation and increased expression of TNF-alpha and IFN-gamma, but not with increased cell death or aminotransferase release. CONCLUSIONS: IL-15 expression increases during liver injury and IL-15 treatment induces a wound healing-type response in healthy adult mice. These findings suggest that IL-15 may contribute to regenerative activity in damaged liver.

   Keywords:
   Animals • Apoptosis • Cell Line • Cell Proliferation • Cell Survival • Cells, Cultured • Gene Expression Regulation • Hepatocytes • Inflammation • Interferon Type II • Interleukin-15 • Liver • Liver Regeneration • Male • Mice • Mice, Inbred C57BL • Mice, Obese • RNA, Messenger • Receptors, Interleukin-15 • Tumor Necrosis Factor-alpha • Wound Healing • drug effects • genetics • genetics* • injuries • metabolism • metabolism* • pathology • pharmacology • physiology*