Publications [#228682] of Daniel P. Kiehart

Papers Published

1. Hutson, MS; Tokutake, Y; Chang, M-S; Bloor, JW; Venakides, S; Kiehart, DP; Edwards, GS, Forces for morphogenesis investigated with laser microsurgery and quantitative modeling., Science (New York, N.Y.), vol. 300 no. 5616 (April, 2003), pp. 145-149 [12574496], [doi]
   (last updated on 2026/01/15)

   Author's Comments:
   *(note Kiehart and Edwards are co-senior and corresponding authors on this paper). Provided a quantitative analysis of relative forces for closure, a phenomenological model for closure and established that forces from zipping at the canthi do not contribute to the bulk sheet movements for closure. Showed that mutant embryos that lack a zygotic contribution of β-integrin fail to zip properly. Work that directly follows from this paper includes an analysis of forces produced by apoptosis (Toyama et al., 2008), a biophysical model for closure (Layton et al., 2009) and more detailed characterization of asymmetries and upregulation of the forces (Layton et al., 2009; Peralta et al., 2007; Peralta et al., 2008).

   Abstract:
   We investigated the forces that connect the genetic program of development to morphogenesis in Drosophila. We focused on dorsal closure, a powerful model system for development and wound healing. We found that the bulk of progress toward closure is driven by contractility in supracellular "purse strings" and in the amnioserosa, whereas adhesion-mediated zipping coordinates the forces produced by the purse strings and is essential only for the end stages. We applied quantitative modeling to show that these forces, generated in distinct cells, are coordinated in space and synchronized in time. Modeling of wild-type and mutant phenotypes is predictive; although closure in myospheroid mutants ultimately fails when the cell sheets rip themselves apart, our analysis indicates that beta(PS) integrin has an earlier, important role in zipping.

   Keywords:
   Animals • Animals, Genetically Modified • Cell Adhesion • Drosophila • Drosophila Proteins • Embryo, Nonmammalian • Embryonic Development • Epithelial Cells • Epithelium • Genes, Insect • Image Processing, Computer-Assisted • Integrin alpha Chains • Integrins • Lasers • Mathematics • Microscopy, Confocal • Microsurgery • Models, Biological* • Morphogenesis* • Mutation • Pseudopodia • embryology* • genetics • physiology • physiology*