Publications [#348250] of Christoph F. Schmidt
search PubMed.Papers Published
- Vermeulen, KC; Wuite, GJL; Stienen, GJM; Schmidt, CF, Optical trap stiffness in the presence and absence of spherical aberrations.,
Applied optics, vol. 45 no. 8
(March, 2006),
pp. 1812-1819 [doi] .
(last updated on 2024/08/25)Abstract:
Optical traps are commonly constructed with high-numerical-aperture objectives. Oil-immersion objectives suffer from spherical aberrations when used for imaging in aqueous solutions. The effect of spherical aberrations on trapping strength has been modeled by approximation, and only a few experimental results are available in the case of micrometer-sized particles. We present an experimental study of the dependence of lateral and axial optical-trap stiffness on focusing depth for polystyrene and silica beads of 2 microm diameter by using oil- and water-immersion objectives. We demonstrate a strong depth dependence of trap stiffness with the oil-immersion objective, whereas no depth dependence was observed with the water-immersion objective.