Publications [#348294] of Christoph F. Schmidt
search PubMed.Papers Published
- deCastro, MJ; Fondecave, RM; Clarke, LA; Schmidt, CF; Stewart, RJ, Working strokes by single molecules of the kinesin-related microtubule motor ncd.,
Nature cell biology, vol. 2 no. 10
(October, 2000),
pp. 724-729 [doi] .
(last updated on 2024/08/25)Abstract:
The ncd protein is a dimeric, ATP-powered motor that belongs to the kinesin family of microtubule motor proteins. Here we resolve single mechanochemical cycles of recombinant, dimeric, full-length ncd, using optical-tweezers-based instrumentation and a three-bead, suspended-microtubule assay. Under conditions of limiting ATP, isolated and transient microtubule-binding events exhibit exponentially distributed and ATP-concentration-dependent lifetimes. These events do not involve consecutive steps along the microtubule, quantitatively confirming that ncd is non-processive. At low loads, a single motor molecule produces ATP-triggered working strokes of about 9 nm, which occur at the ends of binding events.