Publications [#375128] of Christoph F. Schmidt
search PubMed.Papers Submitted
- Daniel Härtter, Lara Haukea, Til Driehorst, Yuxi Long, Guobin Bao, Andreas Primeßnig, Branimir Berecic, Lukas Cyganek, Malte Tiburcy, Christoph F. Schmidt, Wolfram-Hubertus Zimmermann, SarcAsM (Sarcomere Analysis Multi-tool): a comprehensive software tool 2 for structural and functional analysis of sarcomeres in cardiomyocytes,
Nature Biomedical Engineering
(Fall, 2023) .
(last updated on 2024/01/05)Abstract:
Cardiomyocyte function is determined by the structure and dynamics of sarcomeres. To understand how cardiomyocyte function emerges from individual sarcomere dynamics, integrated multiscale analysis, from sarcomeres to the cellular arrangement of myofibrils is essential. Available methods either obtain average sarcomere function from whole-cell dynamics or focus on single structural features. Here we introduce the Sarcomere Analysis Multitool (SarcAsM), that uses machine learning techniques for automated, fast, and unbiased structural assessment of sarcomeric Z-bands, sarcomeres, myofibrils, and sarcomere domains. SarcAsM identifies regions of registered sarcomeres, and tracks and analyzes their average and individual motion. We demonstrate the utility of SarcAsM for the analysis of sarcomere structure and dynamics under acute and chronic drug exposure in real-space (28 nm resolution) and real-time (15 ms resolution) in a custom-generated human induced pluripotent stem cell-derived cardiomyocyte model with fluorescently tagged sarcomere Z-bands. SarcAsM is available as open-access Python package and stand-alone application.