Publications [#349481] of Kathleen M. Pryer
search PubMed.Papers Published
- Windham, MD; Pryer, KM; Poindexter, DB; Li, F; Rothfels, CJ; Beck, JB, A step‐by‐step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited,
Applications in Plant Sciences, vol. 8 no. 4
(2020),
pp. e11342, John Wiley & Sons, Inc. [doi] .
(last updated on 2024/08/25)Abstract:
Premise
Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources-access to a microscope with 1000× magnification is the most limiting factor.Methods and results
Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos.Conclusions
The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.